Coding

Part:BBa_K2239011:Design

Designed by: Ruohong Wang   Group: iGEM17_SDSZ-China   (2017-09-29)
Revision as of 14:39, 29 September 2017 by Registry (Talk | contribs)

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7alpha-HSDH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

The 7alpha-HSDH sequence is cloned from the genome of E.coli through PCR amplification, using the primers designed and synthesized based on its sequence. The restriction site BamH I is added to the upstream primer, and Hind III is added on the downstream primer.

References