Tag

Part:BBa_K2255003:Design

Designed by: Camille Garcia   Group: iGEM17_Aix-Marseille   (2017-08-28)
Revision as of 16:49, 26 September 2017 by Kamy (Talk | contribs) (→‎Design Notes)


Multi-Tag (Rfc 25)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We wanted to create a multi-tag with a his-tag (attachment to Co or Ni column) and a streptavidine-tag (wich can bind biotin). Between those tag we add a TEV clivation site in order to give the possibility of decrease the lengh of this multi-tag.

Our tag is composed of : StrepTag-TEV-6His.

Which can be constructed as : GSG-WSHPQPEL-GSG-ASQFYLNE-GSG-HHHHHH

Glycin and serine residues are added to the sequence in order to give flexibility and space between tags and clivation site.

We retro-translate this sequence and optimized it for E. coli.

Source

This part is made with the consensus sequence of streptavidine tag , histidine tag and TEV protease clivation site.

References