Primer

Part:BBa_K2309031

Designed by: Yijing Gong   Group: iGEM17_XJTLU-CHINA   (2017-09-27)
Revision as of 03:14, 28 September 2017 by Registry (Talk | contribs)

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Standard forward primer for Gibson Assembly to plasmid pNZ8148

This primer contain 18 bp pf BioBrick prefix and the rest for Gibson Assembly. PstI is used to linearize pNZ8148.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 22
    Illegal PstI site found at 17
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 22
    Illegal PstI site found at 17
    Illegal NotI site found at 28
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 22
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 22
    Illegal PstI site found at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 22
    Illegal PstI site found at 17
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None