Composite

Part:BBa_K2235003:Design

Designed by: Achilleas Fardellas, Hrista Micheva   Group: iGEM17_Stockholm   (2017-09-27)
Revision as of 12:38, 27 September 2017 by Registry (Talk | contribs)

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Cumate gene switch expressing BFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 418
    Illegal XhoI site found at 199
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101


Design Notes

There is a multiple cloning site between the two expression cassettes.


Source

Obtained as G-block from IDT and amplified via PCR

References