Coding

Part:BBa_K2255007:Design

Designed by: Camille Garcia   Group: iGEM17_Aix-Marseille   (2017-08-28)
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Signal sequence of p3 from M13 (Rfc25)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Using the logiciel SignalP 4.1, we saw that the cleavage is made between the alanine and the glutamate. T--Aix-Marseille--M13pIII-Sequencesignal.jpeg

In order to gain flexibility, which will help the enzyme to cleave the signal sequence, we add two glycine and one serine residue which we retrotranslate, with the codon biais of E. coli K12.

The signal sequence and D1-D2 sequence are designed to make fusion protein, thus we choose to make them Freiburg assembly standard with Rfc25 prefix and sufix. This will be helpful in order to assemble our biobrick.


Source

This sequence came from the genome of M13.

References