Composite

Part:BBa_M50055:Design

Designed by: Eleanor Glockner   Group: Stanford BIOE44 - S11   (2016-12-11)
Revision as of 06:47, 12 December 2016 by Emrglock (Talk | contribs) (→‎Source)


Atrazine biosensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 760


Design Notes

. We added an eGFP sequence downstream of our atrazine riboswitch so the the riboswitch would control translation of the eGFP.

Source

Riboswitch components: BBa_J89000

Atrazine aptamer: In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine. IJMS International Journal of Molecular Sciences, 15(8), 14332-14347. doi:10.3390/ijms150814332

eGFP sequence: BBa_K598009

References

https://parts.igem.org/Part:BBa_K598009

https://parts.igem.org/Part:BBa_J89000

Williams, R., Crihfield, C., Gattu, S., Holland, L., & Sooter, L. (2014). In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine. IJMS International Journal of Molecular Sciences, 15(8), 14332-14347. doi:10.3390/ijms150814332