Signalling

Part:BBa_M50050:Experience

Designed by: Anthony Agbay   Group: Stanford BIOE44 - S11   (2016-12-11)
Revision as of 17:35, 11 December 2016 by Aja2488 (Talk | contribs) (→‎Conclusions)


Applications of BBa_M50050

  • Standardized growth control for bacteria species that have pathways for sensing and responding to Autoinducer-2.

Stanford Location

Plasmid Name: LuxS Synthase Plasmid

DNA 2.0 Gene #: pD441-CH

Organism: E. coli

Device Type: Actuator

Glycerol Stock Barcode: 0133027189

Box Label: BIOE44 F16

Data

BBa M50050 and BBa M50051 Western Blots.png


BBa M50050 - LuxS-Pink OD600 0 IPTG.png


BBa M50050-DeltaOD600 LuxS-Pink 0 IPTG.png

Conclusions

Western Blot

Based on our analysis of our Western blot, we concluded that we were successful in transfecting our E. coli strain with the correct plasmid and gene sequence. For BBa_M50050, we looked at well sections A and B (BBa_M50050 transfected E. coli at 0 μM and 1000 μM in-solution concentration respectively) compared to the wild-type controls (C and F). What we see is that there are bands of proteins located at the 40 kilodaltons (the anticipated length) in sections A and B, but not in the control sections C and F.

User Reviews

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