Generator
Part:BBa_K2012025
Designed by: Jun Li Group: iGEM16_HZAU-China (2016-10-07)
Revision as of 14:37, 15 October 2016 by Joanna0405 (Talk | contribs)
PcpcG2-172-B0034-sfGFP
sfGFP Generator from pJT119b original plasmid.
Fluorescence assay of CcaS-CcaR system with PcpcG2-172 (BBa_K2012015) in CL1 (△EnvZ), and PCB (△CcaS) as chromophore.
Fluorescence under green light is 1.81-folds of red light, proving that green light activates output expression, the device works well. PcpcG2-172 shows high efficiency.
More importantly, leaked expression in darkness significantly reduced after truncation of the constitutive promoter.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |