Generator

Part:BBa_K2012020

Designed by: Wenqi Huang   Group: iGEM16_HZAU-China   (2016-10-07)
Revision as of 13:48, 16 October 2016 by Johnson Huang (Talk | contribs)


A weak CheZ generator

this generator is altered from BBa_K819010 created by Peking University.Its previous promotor , J23113,is replaced by a slightly stronger promotor J23117. This generator is constructed to detect the relationship between different promoters' strength with their swarming ability. A series of Biobricks were constructed as BBa_K2012018[1], BBa_K2012020[2]

The swarming plate of different E.coli K12 cheZ lacking strain, CL1, transformed with our constructed plamids

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None