Reporter
GG96

Part:BBa_K2145001:Experience

Designed by: Melody Wu, Laura Xinyu Chen, Katie Maruna   Group: iGEM16_Alverno_CA   (2016-10-14)
Revision as of 00:00, 31 October 2016 by Mwu (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.

Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG96 above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR.

Applications of BBa_K2145001

Splates95102.png

Alverno ca 96.png Alverno ca r 96.png Alverno ca g 96.png

User Reviews

UNIQa89207302f0638f4-partinfo-00000000-QINU UNIQa89207302f0638f4-partinfo-00000001-QINU