Reporter
GG95

Part:BBa_K2145000

Designed by: Laura Xinyu Chen, Melody Wu, Katie Maruna   Group: iGEM16_Alverno_CA   (2016-10-13)
Revision as of 22:56, 30 October 2016 by Sclamons (Talk | contribs)


GFP/RFP reporter (Forward/Forward) with random 500bp spacer

This part contains two fluorescent protein expression units (for RFP and GFP) with promoters, RBSs, CDSs, and terminators, both facing forward, with a random 500bp spacer between the two reporters. Parts were amplified from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled with golden gate assembly to make the full part. This composite part is designed to demonstrate the effect, if any, of a 500 bp spacer between genes on supercoiling generated by the two genes, and therefore on interference between the genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2226
    Illegal AgeI site found at 2338
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


[edit]
Categories
Parameters
None