Composite

Part:BBa_K2139015

Designed by: UBC iGEM 2016   Group: iGEM16_British_Columbia   (2016-10-14)
Revision as of 20:38, 30 October 2016 by Dchristy (Talk | contribs)


Endo5a with terminator

E1 catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exoglucanases for the direct conversion of cellulose into monomeric glucose. The protein is monomeric with an optimal temperature and pH over a wide range (unpublished data). The genbank ascension number for this protein is WP_011719450.1 (amino acid) and a PDB code of 1ECE. This construct was codon optimized for E. coli.

Literature data for this part can be found http://aem.asm.org/content/76/19/6360.full

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 975
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 406
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 303


[edit]
Categories
Parameters
None