Coding

Part:BBa_K2139003

Designed by: UBC iGEM 2016   Group: iGEM16_British_Columbia   (2016-10-13)
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Coding Sequence of E1

BBa_K2139003 encompass the coding region for an endo-beta-1,4-glucanase known as E1. E1 catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exoglucanases for the direct conversion of cellulose into monomeric glucose. The polymerization of the protein is monomeric with an optimal temperature and pH over a wide range (unpublished data). The genbank ascension number for this protein is WP_011719450.1 (amino acid) and a PDB code of 1ECE. This construct was codon optimized for E. coli.

Literature data for this part can be found http://aem.asm.org/content/76/19/6360.full

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None