Coding

Part:BBa_K2139001:Experience

Designed by: UBC iGEM 2016   Group: iGEM16_British_Columbia   (2016-10-13)
Revision as of 01:58, 29 October 2016 by Azad (Talk | contribs) (→‎Applications of BBa_K2139001)


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Applications of BBa_K2139001

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Figure 1. (top) Western Blot of C. crescentus cellulase expressing strains, ran on SDS-PAGE and blotted with anti-RsaA. Left to right: (1) Thermofisher ladder, (2) Gluc1C cell lysate, (3) Endo5A cell lysate, (4) E1_399 cell lysate, (5) E1_422 cell lysate, (6) negative control ΔGCSS (ΔrsaA) cell lysate, (7) P4A723 (wildtype) cell lysate, (8) Thermofisher ladder, (9) Gluc1C low pH extracted proteins, (10) Endo5A low pH extracted proteins, (11) E1_399 low pH extracted proteins, (12) E1_422 low pH extracted proteins, (13) ΔGCSS (ΔrsaA) low pH extracted proteins, (14) P4A723 (wildtype) low pH extracted proteins. (bottom) SDS-PAGE stained with coomassie brilliant blue, samples loaded in the same order as above for the Western Blot.

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Figure 2. Assay for cellulase activity with DNPC substrate.

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Figure 3. Cellulase activity assay results for C. crescentus displaying cellulases compared to C. crescentus expressing wildtype RsaA (P4A723).

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