Composite

Part:BBa_K1937002

Designed by: Brice Enjalbert   Group: iGEM16_Toulouse_France   (2016-10-13)
Revision as of 19:53, 29 October 2016 by F lecler (Talk | contribs)


OriKan: a composite part to turn any pSB1C3 into a Bacillus plasmid

Part: BBa_K1937002 (pSB1C3-OriKan)
 (Chassis E. coli/B. subtilis, carrier plasmid pSB1C3)
Length: 2510 bp

While Bacillus subtilis is of huge interest for a growing number of iGEM projects, it is not easy to develop new parts as it is required that they are registered after sub-cloning in the E. coli plasmid pSB1C3. During the iGEM Toulouse 2016 project, we had the idea to create a part that could turn any pSB1C3-based plasmid in a shuttle vector (E. coli/B. subtilis). This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France).

More information available on the design page of the Registry (https://parts.igem.org/Part:BBa_K1937002:Design) and at http://2016.igem.org/Team:Toulouse_France/Description


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2196
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1807
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 522
    Illegal SapI.rc site found at 2020


[edit]
Categories
//plasmidbackbone/component/selectionmarker/antibioticresistance
Parameters
origin
resistance