Measurement

Part:BBa_K2066028

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-06)
Revision as of 23:14, 6 October 2016 by Registry (Talk | contribs)

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pBAD - LacI/Mcherry fusion

This is a Mcherry reporter protein fused to a functional LacI repressor under the control of the arabinose inducible promoter pBAD on the UNS backbone. This part was originally designed to be used as an upstream circuit simulator for WM16_iGEM's circuit control toolbox. The inducible pBad represents an arbitrary circuit with LacI as the product, and as the input to the orthogonal circuit used to modify the transfer function. The mcherry allows for the quantification of the input in the same cell as the quantification of the output (GFP).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1245
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1184
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1019
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1001


[edit]
Categories
Parameters
None