Composite

Part:BBa_K2066055

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-14)
Revision as of 21:37, 16 October 2016 by Jlmaniaci (Talk | contribs)


T7 Promoter without RiboJ Promoter Characterization Part

This part will let us express the entire promoter characterization without riboJ construct in vitro using a T7 in vitro transcription kit, letting us assess the ability of broccoli to fluoresce in the genetic context of our part. Results should be compared to K2066054, which is the same as this part except with the RiboJ sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None