Composite

Part:BBa_K1893005

Designed by: Alyssa Henderson, Aditi Satija   Group: iGEM16_Imperial_College   (2016-10-14)
Revision as of 14:18, 25 October 2016 by Cmoller (Talk | contribs)


Lux receiver with GFP (LuxR+pLux+GFP)

The gene encoding the transcriptional activator LuxR is downstream a constitutive Anderson promoter, j23101. LuxR is activated by O3C6-HSL. Included in the construct is the LuxR-activated promoter pLux upstream a reporter sequence which includes GFP. This part was designed so that we could characterize the LuxR quorum sensing system and determine activation ranges and crosstalk data.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985
    Illegal BsaI.rc site found at 1712


[edit]
Categories
Parameters
None