![](https://parts.igem.org/images/partbypart/icon_intermediate.png)
Intermediate
Part:BBa_K1976050
Designed by: Alana Gouveia, Franziska Hameister, Jonas Sindlinger, Maik Schork Group: iGEM16_TU_Darmstadt (2016-10-14)
Revision as of 03:16, 28 October 2016 by Franny3112 (Talk | contribs)
Colicin E2 DNase domain with B0034 RBS
The colicin DNase domain BBa_K1976048 is fusioned to the strong ribosomal binding site RBSBBa_B0034.![](https://static.igem.org/mediawiki/parts/9/9b/Minicolicin.png)
Figure 1:Colicin E2 DNase domain based on an MD simulation.
Usage
This part can be used for assembly of generators. The So called miniColicin is an active DNase and In combination with the immunity protein Im2 (part part BBa_K1976026) it serves as a safety mechanism (see part BBa_K1976054).
Characteristics
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 46
- 1000COMPATIBLE WITH RFC[1000]
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007
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Categories
Parameters
None |