Intermediate

Part:BBa_K1976050

Designed by: Alana Gouveia, Franziska Hameister, Jonas Sindlinger, Maik Schork   Group: iGEM16_TU_Darmstadt   (2016-10-14)
Revision as of 03:16, 28 October 2016 by Franny3112 (Talk | contribs)

Colicin E2 DNase domain with B0034 RBS

The colicin DNase domain BBa_K1976048 is fusioned to the strong ribosomal binding site RBSBBa_B0034.

Figure 1:Colicin E2 DNase domain based on an MD simulation.


Usage

This part can be used for assembly of generators. The So called miniColicin is an active DNase and In combination with the immunity protein Im2 (part part BBa_K1976026) it serves as a safety mechanism (see part BBa_K1976054).

Characteristics

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 46
  • 1000
    COMPATIBLE WITH RFC[1000]


References
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007

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Parameters
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