Composite

Part:BBa_K1893000:Experience

Designed by: Alyssa Henderson   Group: iGEM16_Imperial_College   (2016-10-14)
Revision as of 20:24, 22 October 2016 by Ach196 (Talk | contribs)


We used this part to engineer a bacterial communication system which was bidirectional, so that two different strains could grow together. Each strain in our system must have a distinct HSL associated with it, so we required two quorum sensing systems which were orthogonal. We found that Las and Rhl had very little crosstalk, indicating that they were good candidates for our system.

This part worked as expected, and when we placed GFP downstream of the LasR promoter, we saw fluorescence when we induced with 3O-C12 AHL.

Applications of BBa_K1893000

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UNIQ7130e451b51e2ac0-partinfo-00000000-QINU UNIQ7130e451b51e2ac0-partinfo-00000001-QINU