Signalling

Part:BBa_K2033012

Designed by: Jimmy Xu   Group: iGEM16_Arizona_State   (2016-10-24)
Revision as of 04:42, 25 October 2016 by Registry (Talk | contribs)

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3-oxo-C6-HSL Sender- EsaI

This is a synthase enzyme that produces 3-oxo-N-[(3S)-2-oxooxolan-3-yl]hexanamide (3-O-C6-HSL). This AHL synthase is designed to be inserted into a modular sender vector BBa_K2033012 with a constitutive Tet promoter, 2 ribosome binding sites (RBSs), an RFC10 prefix and mCherry.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 273
    Illegal NgoMIV site found at 528
    Illegal AgeI site found at 580
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None