![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K2043008:Design
Designed by: Mislav Acman Group: iGEM16_Paris_Bettencourt (2016-10-13)
bpuI-FBD1 laccase codon optimized for E.coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 699
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Bpul-FBD1 sequence was checked and corrected for potential BpiI, BsaI and BsmBI recognition sites. These sites cannot be present in the coding sequence, because the BpiI, BsaI and BsmBI enzymes are widely used in phytobrick cloning (http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks). This BioBrick is the bpul gene (Bba_K2043007) codon optimized for E. coli was improved by addition of fabric binding domain 1 (FBD1) on the 3'-end of the sequence using Golden Gate assembly method.
Source
Bacilus pumillus