Coding
Part:BBa_K2043001:Design
Designed by: Sebastian Sosa-Carrillo and Alicia Calvo-Villamanan Group: iGEM16_Paris_Bettencourt (2016-10-13)
catA from Acinetobacter pittii, codon optimized for E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
His-tag is added in the C-terminal for optimised protein purification catA was codon optimised for E. coli and restriction sites for BsaI were avoided.
Source
Acinetobacter pittii's genome, NCBI Reference Sequence for the gene: YP_004995593.1
References
Lin, J., & Milase, R. N. (2015). Purification and Characterization of Catechol 1, 2-Dioxygenase from Acinetobacter sp. Y64 Strain and Escherichia coli Transformants. The protein journal, 34(6), 421-433.