Reporter

Part:BBa_K1878001

Designed by: John Luke Garcia   Group: iGEM16_Rice   (2016-10-14)
Revision as of 22:43, 14 October 2016 by Registry (Talk | contribs)

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iRFP713 (the fluorescent protein formerly known as "iRFP")

This fluorescent protein has an excitation wavelength of 690 nm and an emission wavelength of 713 nm. The extinction coefficient is 98000 M-1 cm-1. Its quantum yield is 6.3%. Its photostability half-life is 960 seconds. Its pKa is 4.5. Near-infrared fluorescent proteins are good for long-term in vivo imaging and exhibit high brightness in bacteria, mammalian cells, and mice.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 10
    Illegal XhoI site found at 346
    Illegal XhoI site found at 352
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 127
    Illegal NgoMIV site found at 609
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None