Composite

Part:BBa_K1905005

Designed by: Juan Carlos Rueda Silva   Group: iGEM16_TecCEM_HS   (2016-09-13)
Revision as of 04:02, 14 September 2016 by Registry (Talk | contribs)

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Riboswitch to detect E6 mRNA from HPV and express YFP

DNA coding for a riboswitch to detect E6 mRNA from Human Papillomavirus type 16 and type 18 and express Yellow Fluorescent Protein. This part includes a promoter (BB_R0010) and a RBS (BBa_B0034), the reverse complementary for a section of E6 DNA coding region, and the original sequence with one base changed, YFP coding region (BBa_K592010) and a terminator (BBa_B0015). When there is presence of E6 mRNA, the riboswitch turns on and it allows the expression of YFP as reporter protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None