Coding

Part:BBa_K2062005

Designed by: Jaeyoung Jung   Group: iGEM16_ColumbiaU_NYC   (2016-10-14)
Revision as of 00:46, 15 October 2016 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Rhamnosyltransferase 1 [Pseudomonas aeruginosa]

The RhlAB operon codes for mono-rhamnolipid, a virulence factor naturally found in the Pseudomonas Aeruginosa. Our part contains the RhlA and RhlB genes within the operon, coding for the final two enzymes in the mono-rhamnolipid production pathway. Rhamnolipids, consisting of a rhamnose sugar attached to one or multiple lipid side chains, has many uses and has been highlighted by previous iGEM teams as a biosurfactant capable of aiding oil recovery. Another recently shown novel use of rhamnolipids is its mosquito repellent properties.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 69
    Illegal BamHI site found at 629
    Illegal XhoI site found at 805
    Illegal XhoI site found at 2091
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 984
    Illegal NgoMIV site found at 1705
    Illegal NgoMIV site found at 1818
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 294
    Illegal BsaI site found at 1334
    Illegal BsaI.rc site found at 478


[edit]
Categories
Parameters
None