Coding

Part:BBa_K1976049

Designed by: Alana Gouveia, Franziska Hameister, Jonas Sindlinger, Maik Schork   Group: iGEM16_TU_Darmstadt   (2016-10-14)
Revision as of 16:59, 23 October 2016 by Jonassindlinger (Talk | contribs)

Colicin E2 immunity protein

Colicin E2 is a peptide, that is toxic to coliform bacteria. It is one of a group of proteins of this type, it being a nicking endonuclease. It has a corresponding activity inhibiting protein called immunity protein Im2, which is also in the registry under the name .

Figure 1:Structure of Colicin E2 DNase domain derived from a molecular dynamics simulation. Note: this is not actually this part but BBa_K1976048, a 5 amino acids shorter version


Usage

The Colicin E2 immunity protein (BBa_K1976027 and BBa_K1976028) can be used in combination with the Colicin E2 DNase.

Characteristics

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 46
  • 1000
    COMPATIBLE WITH RFC[1000]


References
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007

[edit]
Categories
Parameters
None