Composite

Part:BBa_K2120412

Designed by: Lirong Zhao   Group: iGEM16_BIT-China   (2016-10-13)
Revision as of 08:41, 20 October 2016 by ZLRRR (Talk | contribs)


B0015+tetR+B0034+J23106+ptet+B0032+mazF+B0015

This is one of our application circuit.

This circuit consists of two parts:the inhibitor device and killer device ,which have already been made into basic parts of biobricks.

We choose three different promoters (BBa_J23109,BBa_J23116,BBa_J23106) whose reported strengths are respectively low, medium and strong from constitutive promoter family to express the inhibitor tetR and cI ,and then we construct three plasmids with different copy number containing this circuit, in order to find out the proper promoter which meets the threshold that trigger the killer system in E.coli. According to our results about the lethal effect of killer device , MazF ,so we replace the killer gene with mRFP reporter gene in order to figure out the regulation relationship between two devices.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 851
    Illegal NheI site found at 874
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None