RNA

Part:BBa_K1963011

Designed by: Fatima Ulhuq   Group: iGEM16_Dundee_Schools   (2016-10-14)
Revision as of 18:53, 22 October 2016 by Franksargent (Talk | contribs)


An sRNA system for down-regulation of Serratia marcescens fliC encoding flagellin.

This is a multi-sequence system for small RNA (sRNA) production in an Serratia marcescens host test system - or especially with co-expression of Serratia marcescens Hfq BBa_K1963001. The system is based on the BBa_K1963003 template and has the proD strong promoter, followed by a short antisense sequence targeting the 5' end of the fliC transcript, followed by the S. marcescens chiA hairpin, followed by the strong terminator sequence T1/TE. The anti fliC sRNA is placed after C-193. The system should impair motility of S. marcescens.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101


[edit]
Categories
//awards/part_collection/2016
Parameters
None