Composite

Part:BBa_K1893007:Design

Designed by: Alyssa Henderson, Aditi Satija   Group: iGEM16_Imperial_College   (2016-10-14)
Revision as of 19:45, 22 October 2016 by Ach196 (Talk | contribs)


Cin receiver with GFP (CinR+pCin+GFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 611
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1698


Design Notes

This part does not include an LVA tag on the CinR gene. This means that the protein is not rapidly degraded. When active, the CinR protein binds to the pCin promoter, activating transcription of downstream genes. This part contains everything necessary for O3C14-HSL-induced expression of genes inserted downstream of the pCin promoter, which is, in this case, GFPmut3b. GFPmut3b was included in this part so that we could characterize the activation range of the CinR system.


Source

The CinR gene was synthesised and assembled with the other parts using Biobrick assembly.

References