Composite
Part:BBa_K1961006
Designed by: Yi-Ting Lin Group: iGEM16_HSiTAIWAN (2016-10-11)
Revision as of 08:05, 23 October 2016 by Jonathan0912 (Talk | contribs)
Lead ion detector
This part is designed to detect lead ion following the concept of B. It consists of two parts: (1) PbrR generation:this part produces PbrR which can form a dimer of Pb2+ - PbrR in the presence of Pb2+ . (2) RFP expression: this part is controlled by a promoter(BBa_I721001) which has a binding site of dimer of Pb2+ /PbrR. RFP expression can be observed when the dimer binds onto the promoter.
We redo ligation and do function assay.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1281
Illegal AgeI site found at 1393 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 686
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Categories
Parameters
None |