Composite

Part:BBa_K1893005

Designed by: Alyssa Henderson, Aditi Satija   Group: iGEM16_Imperial_College   (2016-10-14)
Revision as of 19:09, 18 October 2016 by Ach196 (Talk | contribs)


Lux receiver with GFP (LuxR+pLux+GFP) The gene encoding the transcriptional activator RhlR is downstream a constitutive Anderson promoter, j23101. LuxR is activated by O3C6-HSL. Included in the construct is the LuxR-activated promoter pLux upstream a reporter sequence which includes GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985
    Illegal BsaI.rc site found at 1712


[edit]
Categories
Parameters
None