Composite

Part:BBa_K2120308

Designed by: HongYu Yang   Group: iGEM16_BIT-China   (2016-10-13)
Revision as of 19:53, 13 October 2016 by ZLRRR (Talk | contribs)


B0015+LacIq+B0034+pBAD-AraC+plac+B0034+eYFP+B0015

pBAD is a promoter induced by arabinose and arac is the repressor. We use it to test the expression of reporter gene in our validation circuit .And LacIq is an inhibitor which is built to repress the expression of PLac promoter. We use it to control the “on” or ”off” state of toxin gene.YFP is the reporter gene. In order to test these do not interfere with each other , We reserve 【BBa_K2120304 】.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1318
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1483
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1500


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Categories
Parameters
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