Translational_Unit

Part:BBa_K2077002

Designed by: Xintong Xia, Xander Lacrampe, Richard Anthony   Group: iGEM16_RHIT   (2016-08-17)
Revision as of 17:33, 17 August 2016 by Registry (Talk | contribs)

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mls-yeGFP

According to 2015 Rose-Hulman iGEM team, the coding sequence of mitochondrial ribosomal protein mRPS12 starts with a mitochondrial localization signal (mls). From previous research, it is possible that this mls protein is able to transport and direct any attached protein into the mitochondria. To verify the hypothesis brought up in 2015, we constructed a hybrid system with the mls protein and a reporter protein. Instead of using the regular GFP, yeast-enhanced green fluorescent protein (yeGFP) was used in this system as optimal gene expression can be achieved.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 764


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Categories
Parameters
None