Coding

Part:BBa_K2062006

Designed by: Jaeyoung Jung   Group: iGEM16_ColumbiaU_NYC   (2016-10-14)
Revision as of 19:25, 21 October 2016 by Jamesgornet (Talk | contribs)


rhamnosyltransferase 2 [Pseudomonas aeruginosa]

RhlC codes for rhamnosyltransferase 2, which is the last enzyme in the rhamnolipid production pathway. Its function is to convert mono-rhamnolipid to di-rhamnolipid by catalyzing an additional dTDP-L-rhamnose.(1) It was cloned from Pseudomonas aeruginosa PAO1 from an operon also containing a putative fosfomycin resistance protein and putative MFS transporter(2).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 622
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 205
    Illegal NgoMIV site found at 393
    Illegal NgoMIV site found at 931
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 664
    Illegal BsaI.rc site found at 898


[edit]
Categories
Parameters
None