Coding
Part:BBa_K2127002:Design
Designed by: Dawson Zeng Group: iGEM16_IngenuityLab_Canada (2016-10-14)
CP47 His-tagged with Lac Promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 473
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 597
Illegal AgeI site found at 1194
Illegal AgeI site found at 1398 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 918
Illegal SapI site found at 1595
Design Notes
Double Stop Codon. ORF Check
Source
NCBI Reference Sequence: NC_000911.1