Part:BBa_K416001

(Gly4Ser)3 Flexible Peptide Linker

This is a 15 amino acid flexible peptide linker protein domain that is useful for creating functional fusion proteins. The linker is to be fused in frame in between two protein domains, separating the two domains so that they each retain original functions yet will be physically connected.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Improvement by 2016 NCTU_Formosa

In the experiment, we hypothesized that an elongated linker would enhance the function of the fusion protein for the original linker is too short for the proper folding of the two domains. Furthermore, we found no result in the previous history using the Flexible Peptide GS Linker. Therefore, we modified the sequence and added three more amino acids from into the linker. In the result, our improved GS Linker is completely proved by our experiments.

Result and Experiment

       In the experiment, the fusion protein with the elongated GS linker did show the enhanced repellent effect. Observed from the phenomenon, we did prove the enhanced function of the fusion proteins(see Result), so we speculate that the fusion protein has a better secondary structure than the original ones. As a result, we have submitted a basic part for the improved elongated GS linker.

Figure 2.GS linker. The DNA sequence length of GS linker is around 50-60 b.p. In this PCR experiment, the product’s size should be close to 300-350 b.p.