Device

Part:BBa_K2047011

Designed by: Yifei Li   Group: iGEM16_OUC-China   (2016-10-19)
Revision as of 11:10, 19 October 2016 by Registry (Talk | contribs)

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Stem-loop with free energy of -51.4 kcal/mol, measured by Mfold

This sequence encodes a stem loop that can regulate multiple gene expression when inserted in intergenic region followed with a RNaseE site in E.coli. Different stem loops have different regulation effects and we quantitatively weighed this difference by their free energy evaluated by a software---Mfold .Of which the free energy of the most stable secondary structure of this sequence is -20.0 kcal/mol.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 36
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 70
    Illegal PstI site found at 36
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 36
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 36
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None