Composite

Part:BBa_K1955006

Designed by: Justine Hsu   Group: iGEM16_CGU_Taiwan   (2016-10-14)
Revision as of 05:37, 21 October 2016 by Zebrafish (Talk | contribs)

ppSB1C3-5'HYG-OVA-3'UTR

OVA has been commonly used as the antigen for testing the efficiency of antibody response and T cell activation in previous immunology experiments, thus is a easy to use antigen. We add the OVA coding region in between the Leish-5'UTR-HYG and Leish-3'UTR for leishmania to expression our target protein, OVA.





Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1458
    Illegal BamHI site found at 3562
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2689


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Categories
Parameters
None