Part:BBa_K1906000

Elongation Factor Ts

This protein is well known as a translation elongation factor in bacterium Escherichia coli. It can also assemble with phage polymerase domain to form a RNA-dependent-RNA polymerase holoenzyme. It was suggested by XJTLU-CHINA 2016 iGEM team that overexpress of this protein together with EF-Tu are essential for the production of Qbeta replicase holoenzyme.

Characterlization

It was quite surprising that plasmids that carry beta subunit genes don't lead to a heavy production of the protein upon the induction. However, this situation can be improved when EF-Ts and EF-Tu are co-expressed with beta subunit.

Figure 1 is the SDS-PAGE result comparison between cells that transformed with these three proteins and beta subunit alone.

Figure 1. SDS-PAGE result comparison between three-protein-expressing cells and beta subunit-expressing cells, wild-type cells were included as a control group. The gel on the left showing the results of cells that been induced with 2mM IPTG and the gel on the right shows the results of same groups of cells that not been induced. The blue arrow indicates the band position of beta subunit.

It can be clearly seen that cells transformed with beta subunit alone had no detectable level of protein expression. However, the expression of beta subunit can be greatly restored when plasmids carry EF-Tu and EF-Ts are transformed into the cells.

Usage and Biology

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 688
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 487