Composite
Part:BBa_K1955007
Designed by: Chia-Wei Chang Group: iGEM16_CGU_Taiwan (2016-10-16)
E.coli-Leishmania shuttle vector HYG-GFP
A shuttle vector is a vector constructed so that it can reproduce in two different host species. The vector can be quickly amplified in E. coli and then manipulated in Leishmania. This shuttle vector consist of Hygromycin resistant gene as drug selection marker and GFP for easy validation of transfection. It is useful in trying the condition of electroporation of Leishmania since the GFP is easy to observe.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2096
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Categories
Parameters
None |