Composite

Part:BBa_K1955007

Designed by: Chia-Wei Chang   Group: iGEM16_CGU_Taiwan   (2016-10-16)
Revision as of 09:38, 16 October 2016 by Registry (Talk | contribs)

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E.coli-Leishmania shuttle vector HYG-GFP

A shuttle vector is a vector constructed so that it can reproduce in two different host species. The vector can be quickly amplified in E. coli and then manipulated in Leishmania. This shuttle vector consist of Hygromycin resistant gene as drug selection marker and GFP for easy validation of transfection. It is useful in trying the condition of electroporation of Leishmania since the GFP is easy to observe.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2096


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Parameters
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