Composite

Part:BBa_K2120418

Designed by: Lirong Zhao   Group: iGEM16_BIT-China   (2016-10-13)
Revision as of 08:28, 20 October 2016 by ZLRRR (Talk | contribs)


B0015+tetR+B0034+J23116+pTet+B0034+mRFP+B0015


This is one of our application circuit.

Our application circuit circuit consists of two parts:the inhibitor device and killer device ,which have already been made into basic parts of biobricks.

We choose four different promoters whose reported strengths are respectively low, medium,strong and the strongest from constitutive promoter family to express the inhibitor tetR and cI ,and then we construct three plasmid with different copy number containing this circuit, in order to find out the proper promoter which meets the threshold that trigger the killer system.

Go our Parts Registry to learn more.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 851
    Illegal NheI site found at 874
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1528
    Illegal AgeI site found at 1640
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None