DNA

Part:BBa_K2100005

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-10)
Revision as of 01:36, 15 October 2016 by Sarahcaso (Talk | contribs)


pENTR eYFP

eYFP is a fluorescent protein constituitively expressed with the hEF1a (human elongation factor 1 alpha) promoter. The promoter and gene are encoded within an expression vector backbone with an SV40 terminator site after the eYFP gene and an ampicillin resistant gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 222
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 222
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 222
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 222
    Illegal AgeI site found at 5
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection/2016
Parameters
None