Generator

Part:BBa_K2055369

Designed by: Suria Itzel Morales Guzmn   Group: iGEM16_Tec-Monterrey   (2016-10-12)
Revision as of 03:53, 13 October 2016 by Registry (Talk | contribs)

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Na(+)/H(+) antiporter NhaA generator

This construct codes for intermembrane protein Na(+)/H(+) antiporter NhaA, which is a proton pump from E. coli capable of excreting one intracellular Na(+) ion in exchange for two H(+) external protons. This protein is active at alkaline pH. This construct is regulated by weak constitutive promoter and its objective is to augment bacterial resistance to alkaline pH.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 415
    Illegal BamHI site found at 1120
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 382
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None