Composite

Part:BBa_K1947029

Designed by: Xuechun Wang   Group: iGEM16_NEFU_China   (2016-10-14)
Revision as of 14:16, 14 October 2016 by Yukiwang (Talk | contribs)

This part is used to improve the protein purification.

TEV protease may bring some impure proteins. We use intein to replace the TEV site. The M. xenopi GyrA intein provides a paradigm for a minimal protein splicing element. Intein can be cleaved by DTT. DTT as reducing agent protects the free thiol groups of the protein from oxidation and so it doesn’t bring foreign substance. As a result, intein can achieve a better effect of purification than TEV protease.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1230
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1150


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Categories
Parameters
None