Coding

Part:BBa_K1974020

Designed by: YU-CHUN WU   Group: iGEM16_NCTU_Formosa   (2016-10-13)
Revision as of 00:06, 20 October 2016 by PennyWu (Talk | contribs)


Snowdrop lectin+linker+6X His-Tag

Introduction:

Figure 1.snowdrop lectin+linker+6xHistag

      Snowdrop (Galanthus nivalis) lectin is a protein that has special affinity to the glycoprotein on the surface of insect epithelial cells. According to reference, snowdrop-lectin is resistant to high temperature and would not be degraded by digestive juice. The species-specificity is based on the toxin, and the snowdrop lectin is the role of the carrier. When it is ligated by Pantide, which can increase the oral activity of Pantide.

Figure 2.. Snowdrop lection The DNA sequence length of Snowdrop lectin is around 300-400 b.p. In this PCR experiment, the product’s size should be close to 550-700 b.p.
Figure 3. Mechanism: In the alimentary canal, lectin binds to gut epithelial glycoproteins, and crosses the epithelium cell by endocytosis and exocytosis, and transported into haemolymph.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None