Coding

Part:BBa_K2010007

Designed by: William Cho   Group: iGEM16_Harvard_BioDesign   (2016-09-16)
Revision as of 07:27, 8 October 2016 by Williamcho (Talk | contribs)


pelB + sfGFP + PETase (PET-degrading enzyme, origin I. sakaiensis)

PETase is the PET(poly(ethylene terephtalate))-degrading enzyme first identified in Ideonella sakaiensis. This sequence is the E. coli K12 optimized DNA sequence for PETase.

This part includes a T7 promoter (BBa_I712074), RBS 34 (BBa_B0034), and PETase fused with a His-tag, for purification. In addition, this part includes sfGFP, a variant of GFP that facilitates protein folding, and pelB, a secretion tag to secrete PETase to the periplasm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 118
    Illegal NgoMIV site found at 815
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1233


[edit]
Categories
//function/degradation
Parameters
protein