Composite
RBS + ButC
Part:BBa_K2052015:Design
Designed by: Burak Kızıl, Seniz Yuksel Group: iGEM16_METU_HS_Ankara (2016-09-24)
ButCoat with strong RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
RBS and our protein coding region (ButCoaT) are digested from our whole construct with the enzymes Sall and HindIII.
In order to be expressed in E.coli BL21, the sequence was optimized with the use of codon bias rule and the unwanted restriction cut sides(SalI, HindIII) were removed with point mutation.
Source
Source of ButCoaT Gene: Roseburia intestinalis L1-82