Reporter

Part:BBa_K2150017:Design

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-12)
Revision as of 00:16, 20 October 2016 by Freedom.H@UCAS (Talk | contribs) (→‎Design Notes)


pT7 TetX-GFP(fusion protein)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 608
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1905


Design Notes

Expression level of tetX can be measured by the fluorescence intensity of GFP and the expression of tetracycline-degrading enzyme tetX can be amplified by T7 RNA polymerase(not included here).

Source

pT7 promoter with RBS comes from BBa_K525998,DT from BBa_B0015. As for fusion protein tetX-GFP, tetX is the same as BBa_K2150101, the protein linker is provided by our advisor Li Hua, and the GFP is from BBa_E0040. We combine this three parts using overlap extension polymerase chain reaction (OE-PCR).

References