Coding

Part:BBa_K2118001

Designed by: Laura Ros-Freixedes   Group: iGEM16_UPF-CRG_Barcelona   (2016-10-14)
Revision as of 23:51, 19 October 2016 by Nuriaarmengol (Talk | contribs)


patA gene

Putrescine aminotransferase gene, also called PATase or PatA, is an original gene from Escherichia Coli. It encodes for a protein which is involved in polyamine degradation, specifically putrescine. It catalyses the first step in one of two pathways for the degradation of this polyamine. It carries out the aminotransferase aminotransferase reaction from putrescine to 2-oxoglutarate which leads to glutamate and 4-aminobutanal. This last compound spontaneously cyclizes to form 1-pyrroline.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 386
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 702
    Illegal AgeI site found at 843
    Illegal AgeI site found at 858
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/probiotics/production
Parameters
None